Journal: The American Journal of Pathology

Article Title: Deletion of the Mitochondrial Complex-IV Cofactor Heme A:Farnesyltransferase Causes Focal Segmental Glomerulosclerosis and Interferon Response

doi: 10.1016/j.ajpath.2018.08.018

Figure Lengend Snippet: Cox10 deficiency in the nephron leads to tubulointerstitial damage. A: Representative photomicrographs taken from periodic acid-Schiff (PAS)–stained kidney sections of Cox10Δ/Δ and Cox10+/+ mice at 3 months of age. Using PAS-stained kidney sections, tubular damage was evaluated on the basis of the percentage of dilated, atrophic tubules (T); tubules were in Cox10Δ/Δ compared with Cox10+/+ kidney. B–F: Evaluation of fibrosis (B), activated fibroblasts (C), proximal tubular injury (D), apoptosis (E), and macrophage infiltration in kidneys (F) from Cox10Δ/Δ and Cox10+/+ mice at 3 months of age using immunostaining. All statistics analyzed using the U-test. Data are expressed as means ± SEM (A–F). n = 4 to 9 (A and B); n = 3 (C–F). ∗P ≤ 0.05, ∗∗P ≤ 0.01. Scale bar = 100 μm (A). Original magnification, ×20 (B–F). KIM-1, kidney injury molecule-1; LTL, lotus tetragonolobus lectin; α-SMA, α-smooth muscle actin.

Article Snippet: Cryostat-cut mouse kidney sections (5 μm thick) were stained for the following: fibrosis, using Cy3-conjugated anti–α-smooth muscle actin antibody (Ab;; 1:200; clone 1A4; Sigma-Aldrich, St. Louis, MO); tubular injury, using polyclonal rabbit anti-human kidney injury molecule-1 Ab (1:200; catalog ab47635; Abcam, Cambridge, MA); apoptosis, using rabbit anti-human cleaved caspase-3 Ab (1:50; clone 5A1E; Cell Signaling Technology, Danvers, MA); proximal tubules, using fluorescein isothiocyanate–conjugated lotus tetragonolobus lectin (LTL; 1:200; catalog FL-1321; Vector Laboratories, Burlingame, CA); and macrophages, using rat anti-mouse F4/80 Ab (1:400; clone BM-8; Thermo Fisher Scientific).

Techniques: Staining, Immunostaining

Journal: Biomolecules

Article Title: Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

doi: 10.3390/biom14040499

Figure Lengend Snippet: Demographic, clinical, and biological data of patients with type 2 DM and healthy controls.

Article Snippet: The biomarkers studied were assessed by the ELISA technique, as follows: podocyte injury biomarkers synaptopodin (Catalogue Nr. abx055120 Abbexa, Cambridge, UK; sensitivity—0.10ng/mL, detection range—0.156–10 ng/mL, coefficient of variance (CV) < 10%) and podocalyxin (Catalogue Nr. E-EL-H2360 Elabscience, Houston, TX, USA; sensitivity—0.1 ng/mL, detection range—0.16–10 ng/mL, coefficient of variance (CV) < 10%); proximal tubule (PT) dysfunction biomarkers kidney injury molecule—1(KIM-1, Catalogue Nr. E-EL-H6029 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%) and N-acetyl-β-(D)-glucosaminidase (NAG, Catalogue Nr. E-EL-H0898 Elabscience, Houston, TX, USA; sensitivity—0.94 ng/mL, detection range—1.56–100 ng/mL, CV < 10%); biomarkers of inflammation: tumor necrosis alpha (TNFα, Catalogue Nr. E-EL-H0109 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%); intercellular adhesion molecule (ICAM-1, Catalogue Nr. E-EL-H6114 Elabscience, Houston, TX, USA; sensitivity—0.19 ng/mL, detection range—0.31–20 ng/mL, CV < 10%); interleukins [IL-17A (Catalogue Nr. E-EL-H0105 Elabscience, Houston, TX, USA; sensitivity—18.75 pg/mL, detection range—31.25–2000 pg/mL, CV < 10%), IL-18 (Catalogue Nr. E-EL-H0253 Elabscience, Houston, TX, USA; sensitivity—9.38 pg/mL, detection range—15.63–1000 pg/mL, CV < 10%), and IL-10 (Catalogue Nr. E-EL-H6154 Elabscience, Houston, TX, USA; sensitivity—0.94 pg/mL, detection range—1.56–100 pg/mL, CV < 10%)].

Techniques:

Journal: Biomolecules

Article Title: Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

doi: 10.3390/biom14040499

Figure Lengend Snippet: Multivariable regression analysis for TNFα and ICAM-1 with mtDNA, biomarkers of podocyte injury and PT dysfunction.

Article Snippet: The biomarkers studied were assessed by the ELISA technique, as follows: podocyte injury biomarkers synaptopodin (Catalogue Nr. abx055120 Abbexa, Cambridge, UK; sensitivity—0.10ng/mL, detection range—0.156–10 ng/mL, coefficient of variance (CV) < 10%) and podocalyxin (Catalogue Nr. E-EL-H2360 Elabscience, Houston, TX, USA; sensitivity—0.1 ng/mL, detection range—0.16–10 ng/mL, coefficient of variance (CV) < 10%); proximal tubule (PT) dysfunction biomarkers kidney injury molecule—1(KIM-1, Catalogue Nr. E-EL-H6029 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%) and N-acetyl-β-(D)-glucosaminidase (NAG, Catalogue Nr. E-EL-H0898 Elabscience, Houston, TX, USA; sensitivity—0.94 ng/mL, detection range—1.56–100 ng/mL, CV < 10%); biomarkers of inflammation: tumor necrosis alpha (TNFα, Catalogue Nr. E-EL-H0109 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%); intercellular adhesion molecule (ICAM-1, Catalogue Nr. E-EL-H6114 Elabscience, Houston, TX, USA; sensitivity—0.19 ng/mL, detection range—0.31–20 ng/mL, CV < 10%); interleukins [IL-17A (Catalogue Nr. E-EL-H0105 Elabscience, Houston, TX, USA; sensitivity—18.75 pg/mL, detection range—31.25–2000 pg/mL, CV < 10%), IL-18 (Catalogue Nr. E-EL-H0253 Elabscience, Houston, TX, USA; sensitivity—9.38 pg/mL, detection range—15.63–1000 pg/mL, CV < 10%), and IL-10 (Catalogue Nr. E-EL-H6154 Elabscience, Houston, TX, USA; sensitivity—0.94 pg/mL, detection range—1.56–100 pg/mL, CV < 10%)].

Techniques:

Journal: Biomolecules

Article Title: Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

doi: 10.3390/biom14040499

Figure Lengend Snippet: Univariable regression analysis for cerebral hemodynamic indices with albuminuria, eGFR, and biomarkers of podocyte injury and PT dysfunction.

Article Snippet: The biomarkers studied were assessed by the ELISA technique, as follows: podocyte injury biomarkers synaptopodin (Catalogue Nr. abx055120 Abbexa, Cambridge, UK; sensitivity—0.10ng/mL, detection range—0.156–10 ng/mL, coefficient of variance (CV) < 10%) and podocalyxin (Catalogue Nr. E-EL-H2360 Elabscience, Houston, TX, USA; sensitivity—0.1 ng/mL, detection range—0.16–10 ng/mL, coefficient of variance (CV) < 10%); proximal tubule (PT) dysfunction biomarkers kidney injury molecule—1(KIM-1, Catalogue Nr. E-EL-H6029 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%) and N-acetyl-β-(D)-glucosaminidase (NAG, Catalogue Nr. E-EL-H0898 Elabscience, Houston, TX, USA; sensitivity—0.94 ng/mL, detection range—1.56–100 ng/mL, CV < 10%); biomarkers of inflammation: tumor necrosis alpha (TNFα, Catalogue Nr. E-EL-H0109 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%); intercellular adhesion molecule (ICAM-1, Catalogue Nr. E-EL-H6114 Elabscience, Houston, TX, USA; sensitivity—0.19 ng/mL, detection range—0.31–20 ng/mL, CV < 10%); interleukins [IL-17A (Catalogue Nr. E-EL-H0105 Elabscience, Houston, TX, USA; sensitivity—18.75 pg/mL, detection range—31.25–2000 pg/mL, CV < 10%), IL-18 (Catalogue Nr. E-EL-H0253 Elabscience, Houston, TX, USA; sensitivity—9.38 pg/mL, detection range—15.63–1000 pg/mL, CV < 10%), and IL-10 (Catalogue Nr. E-EL-H6154 Elabscience, Houston, TX, USA; sensitivity—0.94 pg/mL, detection range—1.56–100 pg/mL, CV < 10%)].

Techniques:

Journal: Biomolecules

Article Title: Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

doi: 10.3390/biom14040499

Figure Lengend Snippet: Univariable regression analysis for the cerebral hemodynamic indices with mtDNA and biomarkers of inflammation.

Article Snippet: The biomarkers studied were assessed by the ELISA technique, as follows: podocyte injury biomarkers synaptopodin (Catalogue Nr. abx055120 Abbexa, Cambridge, UK; sensitivity—0.10ng/mL, detection range—0.156–10 ng/mL, coefficient of variance (CV) < 10%) and podocalyxin (Catalogue Nr. E-EL-H2360 Elabscience, Houston, TX, USA; sensitivity—0.1 ng/mL, detection range—0.16–10 ng/mL, coefficient of variance (CV) < 10%); proximal tubule (PT) dysfunction biomarkers kidney injury molecule—1(KIM-1, Catalogue Nr. E-EL-H6029 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%) and N-acetyl-β-(D)-glucosaminidase (NAG, Catalogue Nr. E-EL-H0898 Elabscience, Houston, TX, USA; sensitivity—0.94 ng/mL, detection range—1.56–100 ng/mL, CV < 10%); biomarkers of inflammation: tumor necrosis alpha (TNFα, Catalogue Nr. E-EL-H0109 Elabscience, Houston, TX, USA; sensitivity—4.69 pg/mL, detection range—7.81–500 pg/mL, CV < 10%); intercellular adhesion molecule (ICAM-1, Catalogue Nr. E-EL-H6114 Elabscience, Houston, TX, USA; sensitivity—0.19 ng/mL, detection range—0.31–20 ng/mL, CV < 10%); interleukins [IL-17A (Catalogue Nr. E-EL-H0105 Elabscience, Houston, TX, USA; sensitivity—18.75 pg/mL, detection range—31.25–2000 pg/mL, CV < 10%), IL-18 (Catalogue Nr. E-EL-H0253 Elabscience, Houston, TX, USA; sensitivity—9.38 pg/mL, detection range—15.63–1000 pg/mL, CV < 10%), and IL-10 (Catalogue Nr. E-EL-H6154 Elabscience, Houston, TX, USA; sensitivity—0.94 pg/mL, detection range—1.56–100 pg/mL, CV < 10%)].

Techniques: